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1. Which of the following is used to determine the appropriate stringency (Wallace rule)?
(A): Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)
(B): Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
(C): Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
(D): Tm = 69.3° + 0.41 (%)G+C -650/1
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