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You Are On Multi Choice Question Bank SET 160
8001. Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?
(A): all of these
8002. In animal cell culture, particularly mammalian cell culture, transformation means
(A): both (a) and (b) (B): phenotypic modifications of cells in culture (C): release of genetic information (D): uptake of new genetic material
8003. How does CO2 help in the cell metabolism during cell culture?
(A): All of the above (B): For monitoring pH of the culture (C): Helps in the cells respiration (D): It participates in the de novo synthesis of purines and pyrimidines
8004. Which of the following is not the explantation technique?
(A): Adherent primary culture (B): Carrel flask culture (C): Roller test tube culture (D): Slide culture
8005. Cells which have undergone transformation frequently become
(A): anchorage dependent (B): anchorage independent (C): stable (D): unstable
8006. Range of optimum glucose concentration present in the culture media is
(A): 105-150 mmol/litre (B): 5.5 - 55 mmol/litre (C): 55 - 75 mmol/litre (D): 75-105 mmol/litre
8007. The technique of organ culture may be divided on the basis of employing
(A): both (a) and (b) (B): liquid medium (C): semi-solid medium (D): solid medium
8008. At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via
(A): anaerobic pathway (B): both (a) and (b) (C): none of these (D): oxidative pathway
8009. An established cell line can be called where it has been subcultured at least
(A): 40 times at an interval of 3 days between subcultures (B): 50 times at an interval of 3 days between subcultures (C): 70 times at an interval of 1 day between subcultures (D): 70 times at an interval of 3 days between subcultures
8010. Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)
(A): 0.05-5 nmol of O2/cell/h (B): 0.05-5 pmol of O2/cell/h (C): 5 - 10 nmol of O2 /cell/h (D): 5 - 10 pmol of O2/cell/h
8011. Which of the following is not a source of energy in active muscle cells?
(A): ATP (B): Creatine phosphate (C): Glucose (D): Lactic acid
8012. Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called
(A): established cell lines (B): primary cell lines (C): propagated cell lines (D): secondary cell lines
8013. Higher dissolved oxygen concentration in the culture media are toxic and lead to
(A): all of the above (B): DNA degradation (C): lipid peroxidation (D): metabolism of nutrients in culture media at a rate greater than that required for consumption
8014. Which of the following is the technique used for the embryo culture?
(A): All of these (B): Organ cultures on agar (C): Organ cultures on plasma clots (D): Whole embryo cultures
8015. The major problem associated with the isolation of free cells and cell aggregates from organs is that of
(A): disintegrating the cells from their supporting matrix (B): inhibiting the cells from their supporting matrix (C): none of the above (D): releasing the cells from their supporting matrix
8016. For culturing, plasma from the adult chicken is preferred to mammalian plasma because
(A): it forms a clear and solid coagulum even after dilution (B): it forms a semi solid coagulum (C): it is too opaque
8017. Toxicity due to accumulation of ammonia can be overcome
(A): all of the above (B): by controlled addition of glutamine at low level (C): by removal of ammonia or ammonium from culture medium (D): by substituting glutamine by glutamate
8018. Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is
(A): 150-300 (B): 280-360 (C): 300-325 (D): 360-400
8019. Disaggregating of cells can be achieved by
(A): all of the above (B): enzymatic digestion (C): physical disruption (D): treating with chelating agents
8020. Accumulation of lactate leads to
(A): increase in pH (B): no change in pH (C): no loss of cell viability (D): reduction in the pH of culture hence loss of cell viability
8021. The person who is said to be the “Iron man” of India is:
(A): Bhagat Singh (B): Sardar Vallabhbhai Patel (C): Subhash Chandra Bose (D): V.K. Krishna Menon
8022. സിംല കരാർ ഒപ്പുവെച്ച വർഷമേത് ? [Simla karaar oppuveccha varshamethu ?]
(A): 1971 (B): 1972 (C): 1975 (D): 1977
8023. ബേബി ഫ്രണ്ട് ലി സ്റ്റേറ്റ് എന്നറിയപ്പെടുന്ന ഇന്ത്യന് സംസ്ഥാനം ? [Bebi phrandu li sttettu ennariyappedunna inthyanu samsthaanam ?]
(A): ആസാം [Aasaam] (B): കേരളം [Keralam] (C): ഗോവ [Gova] (D): ബീഹാര് [Beehaaru ]
8024. Granary of Kerala:
(A): Kochi (B): Kollam (C): Kuttanad (D): Palakkad
8025. Mark is the currency of:
(A): Egypt (B): Germany (C): Japan (D): Qatar
8026. At acidic pH, penicillin remains in
(A): aqueous phase (B): both (a) and (b) (C): precipitates (D): solvent phase
8027. Chlorotetracyclin is soluble in
(A): all of these (B): ether (C): organic solvents (D): water
8028. Penicillin is produced by
(A): aerobic fermentation (B): aerobic fermentation followed by anaerobic fermentation (C): anaerobic fermentation (D): anaerobic fermentation followed by aerobic fermentation
8029. Penicillin is recovered after fermentation as
(A): calcium penicillin (B): penicillin only (C): potassium penicillin (D): sodium penicillin
8030. Media composition for the production of streptomycin is
(A): soybean meal, glucose, peptone, malt extract, ammonium chloride (B): soybean meal, glucose, peptone, malt extract, ammonium sulphate (C): soybean meal, glucose, peptone, malt extract, calcium carbonate (D): soybean meal, glucose, peptone, malt extract, sodium chloride
8031. Which of the following organism(s) produce(s) tetracycline?
(A): All of these (B): Nocardia sulphurea (C): Steptomyces aureofaciens (D): Steptomyces ramosus
8032. Media used for the production of chlorotetracyclin consists of
(A): corn steep liquor, CaCO3 and (NH4)2SO4 (B): corn steep liquor, CaCO3, (NH4)2SO4 and NH4 Cl (C): sugar, CaCO3 and (NH4)2SO4 (D): sugar, corn steep liquor, CaCO3, (NH4)2SO4 and NH4 Cl
8033. Which of the following precursor is added in the medium to get penicillin G?
(A): Ammonium chloride (B): Ammonium sulphate (C): Phenyl acetic acid (D): Phenyl carbamic acid
8034. High yield of chlorotetracyclin requires
(A): aeration which does not affect the yield (B): continuous aeration (C): controlled aeration (D): no aeration
8035. Streptomycin is produced by
(A): S. aerofaciens (B): S. griseoflavus (C): S. griseus (D): S. ramosus
8036. Which of the following event occurs during second phase of growth of P. chrysogenum?
(A): Both (a) and (b) (B): Mycelial mass increases (C): pH increase (D): Synthesis of penicillin is high
8037. Inoculum preparation for the fermentation medium for penicillin takes place on
(A): all of these (B): barley seeds (C): rice seeds (D): wheat seeds
8038. Antibiotics are typically produced in fed batch reactors because
(A): All of the above (B): antibiotic yields are generally higher when cell growth slows (C): antibiotic yields are generally higher when cells enter the stationary phase (D): the precursors are often toxic to the cells
8039. Which of the following event occurs during third phase of growth of P chrysogenum?
(A): All of the above (B): Autolysis of the medium starts (C): Concentration of antibiotic increases in the medium (D): Slight rise in pH due to liberation of ammonia
8040. Vegetable oil, (corn oil or soybean oil) added in the fermentation medium for penicillin, act as
(A): antifoam agent (B): both (a) and (c) (C): carbon source (D): source of nutrients
8041. Which of the following changes occur during first phase of growth of Penicillium chrysogenum?
(A): All of the above (B): Ammonia is liberated in the medium (C): Growth of mycelia occurs (D): Lactic acid present in the corn steep liquor is utilized at the maximum rate
8042. The yield of the antibiotic depends upon
(A): age of the inoculum (B): all of these (C): composition of the medium (D): pH of the medium
8043. During which phase of growth of Penicillium chrysogenum maximum antibiotic production takes place
(A): during the first phase (B): during the second phase (C): during the third phase (D): same in all the phases
8044. Fermentation medium for oxytetracyclin (terramycin) consist of
(A): CSL, (NH4)2 SO4, sodium chloride and CaCO3 (B): CSL, starch, (NH4)2 SO4, sodium chloride and CaCO3 (C): CSL, starch,(NH4)2 SO4, ammonium chloride and CaCO3 (D): CSL,(NH4)2 SO4, ammonium chloride and CaCO3
8045. pH of the fermentation medium for chlorotetracyclins is
(A): 5-6 (B): 6-7 (C): 7-8 (D): 8-9
8046. At normal pH, penicillin remains in
(A): aqueous phase (B): both (a) and (b) (C): precipitates (D): solvent phase
8047. The name given to a disease of the eye, caused by increased tension within the eyeball is:
(A): Cataract (B): Colour blindness (C): Conjunctivitis (D): Glaucoma
8048. The most abundant substance on Earth is
(A): carbon (B): metals (C): soil (D): water
8049. Who was the chief architect of Prarthana Samaj ?
(A): Bal Gangadhar Tilak (B): Keshab Chandra Sen (C): Mahadev Gobind Ranade (D): R.G. Bhandarkar
8050. ക്രിക്കറ്റിന്റെ ഉത്ഭവം ഏതു രാജ്യത്തായിരുന്നു ? [Krikkattinte uthbhavam ethu raajyatthaayirunnu ?]
(A): ഓസ്ട്രേലിയ [ osdreliya ] (B): ഇംഗ്ലണ്ട് [Imglandu ] (C): ന്യൂസിലാൻഡ് [Nyoosilaandu ] (D): ശ്രീലങ്ക [Shreelanka]
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